Whitening activity of luteolin related to the inhibition of cAMP pathway in α-MSH-stimulated B16 melanoma cells

To examine the possibility of luteolin as a whitening agent, we measured antioxidant activity using DPPH assay, NBT/XO assay and intracellular ROS scavengning assay and depigmenting activity using tyrosinase assay, α-MSH-induced melanin production in B-16 cells. Luteolin showed dose-dependent anti-o...

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Bibliographic Details
Published in:Archives of pharmacal research 2008, 31(9), , pp.1166-1171
Main Authors: Choi, Mi Young, Song, Ho Sun, Hur, Hyun Sook, Sim, Sang Soo
Format: Article
Language:English
Subjects:
alpha-MSH - pharmacology
Arbutin - pharmacology
Biphenyl Compounds
Cell Survival - drug effects
Colforsin - pharmacology
Cyclic AMP - physiology
Enzyme-Linked Immunosorbent Assay
Free Radical Scavengers - pharmacology
Humans
Luteolin - pharmacology
Medicine
Melanins - antagonists & inhibitors
Melanins - biosynthesis
Melanoma, Experimental - metabolism
Monophenol Monooxygenase - antagonists & inhibitors
Pharmacology/Toxicology
Pharmacy
Picrates - metabolism
Reactive Oxygen Species - metabolism
Signal Transduction - drug effects
Superoxides - metabolism
Xanthine Oxidase - antagonists & inhibitors
Xanthine Oxidase - metabolism
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Summary:To examine the possibility of luteolin as a whitening agent, we measured antioxidant activity using DPPH assay, NBT/XO assay and intracellular ROS scavengning assay and depigmenting activity using tyrosinase assay, α-MSH-induced melanin production in B-16 cells. Luteolin showed dose-dependent anti-oxidant activity in DPPH, NBT/XO and intracellular ROS assay. Also, luteolin directly inhibited xanthine oxidase activity in a dose-dependent manner. Although luteolin did not directly inhibit tyrosinase activity, it dose-dependently inhibited both tyrosinase activity and melanin production in B16 melanoma cells stimulated by 1 μM α-MSH. Luteolin dose-dependently inhibited cAMP levels in B16 melanoma cells stimulated by 1 μM α-MSH and 1 μM forskolin, which suggest that luteolin directly inhibits adenyl cyclase in B16 melanoma cells. Therefore, these results suggest that whitening activity of luteolin may be due to the inhibition of adenyl cyclase involved in the signal pathway of α-MSH in B16 melanoma cells.
ISSN:0253-6269
1976-3786
DOI:10.1007/s12272-001-1284-4