Fluorescence immunoassay of anti-cyclic citrulinated peptide (CCP) autoantibodies by using parylene-H film

A fluorescence immunoassay for rheumatoid arthritis was presented by immobilizing a short peptide cyclic citrullinated peptide (CCP) through parylene-H film. The parylene-H film is a polymer of pxylene modified with formyl groups which can react with primary amine groups of proteins or peptides. In...

Full description

Saved in:
Bibliographic Details
Published in:Biochip journal 2011, 5(3), , pp.242-245
Main Authors: Ko, Hyuk, Lee, Ga-yeon, Jeon, Byoung-Jin, Pyun, Jae-Chul
Format: Article
Language:English
Subjects:
Arthritis
Autoantibodies
Biomedical Engineering and Bioengineering
Biotechnology
Chemistry
Chemistry and Materials Science
Citrulline
Coupling
Fluorescence
Immobilization
Immunoassay
Original Research
Peptides
Polymer films
Reagents
Rheumatoid arthritis
생물공학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A fluorescence immunoassay for rheumatoid arthritis was presented by immobilizing a short peptide cyclic citrullinated peptide (CCP) through parylene-H film. The parylene-H film is a polymer of pxylene modified with formyl groups which can react with primary amine groups of proteins or peptides. In this work, the covalent coupling of the peptide can be produced by only one step of incubation without additional coupling reagent, and the immobilization efficiency to the parylene-H film was compared with the immobilization of CCP through conventional physical adsorption. The applicability of this immobilization method for short peptides is demonstrated by detecting autoantibodies in rheumatoid arthritis patient serum.
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-011-5308-8