Efficient expression of myo-inositol oxygenase in Escherichia coli and application for conversion of myo-inositol to glucuronic acid

Glucuronic acid is an important biochemical with wide applications in the food and medical industries. The myo-inositol oxygenase (MIOX) gene was synthesized and expressed in Escherichia coli BL 21(DE3). After optimization of induction conditions and the culture temperature, the highest MIOX activit...

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Bibliographic Details
Published in:Food science and biotechnology 2014, 23(2), , pp.445-450
Main Authors: Zheng, Shuxiang, Wei, Peilian, Huang, Lei, Cai, Jin, Xu, Zhinan
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Escherichia coli
Food Science
Nutrition
Research Article
식품과학
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Summary:Glucuronic acid is an important biochemical with wide applications in the food and medical industries. The myo-inositol oxygenase (MIOX) gene was synthesized and expressed in Escherichia coli BL 21(DE3). After optimization of induction conditions and the culture temperature, the highest MIOX activity (45.46 kU/L) was achieved when 0.1 mM isopropyl-thio-β- d -galactoside (IPTG) was added to cell cultures with an OD 600 value 1.0 followed by induction at 26°C for 8 hours. The purified MIOX enzyme exerted characteristics similar to the native form. Conversion of myo-inositol to glucuronic acid was performed using whole cells in a pH 8.5 buffer system. Whole cells harboring myo-inositol oxygenase were used as a biocatalyst to produce 2.13 g/L of glucuronic acid with a conversion rate of 99%. A promising novel process for glucuronic acid production from abundant agricultural byproducts is presented.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-014-0061-0