Antioxidant activity of phenolic compounds from canola (Brassica napus) seed

The antioxidant activities of various extracts from canola ( Brassica napus ) seed were investigated using the DPPH assay, ABTS radical assay, and reducing power. An 80% methanol extract from canola seed was sequentially fractionated and separated according to the solvent polarity and Sephadex LH-20...

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Bibliographic Details
Published in:Food science and biotechnology 2014, 23(6), , pp.1753-1760
Main Authors: Jun, Hyun-Il, Wiesenborn, Dennis P., Kim, Young-Soo
Format: Article
Language:English
Subjects:
Brassica napus
Chemistry
Chemistry and Materials Science
Food Science
Nutrition
Research Article
식품과학
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Summary:The antioxidant activities of various extracts from canola ( Brassica napus ) seed were investigated using the DPPH assay, ABTS radical assay, and reducing power. An 80% methanol extract from canola seed was sequentially fractionated and separated according to the solvent polarity and Sephadex LH-20 chromatography, respectively. The antioxidant activities of various extracts and their total phenolic content (TPC) and total flavonoid content (TFC) were closely correlated, resulting in correlation coefficient values higher than 0.87. Of all extracts, the sub-fraction 3 had the highest TPC (462.3 μg/mg) and TFC (75.4 μg/mg) while it showed the lowest EC 50 value (183.1 μg/mL). The EC 50 value of sub-fraction 3 measured using the DPPH radical assay was 1.3 times lower than that of butylated hydroxytoluene (BHT). The major free phenolic compound was trans -sinapic acid (193.4 μg/mg) and sinapic acid derivatives identified as 1- O -β- d -glucopyranosyl sinapate and 1,2-di- O -sinapoyl-β- d -glucose in sub-fraction 3.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-014-0240-z