Synergistic antimicrobial activity of bacteriophages and antibiotics against Staphylococcus aureus

This study was designed to assess the synergistic antimicrobial effect of phages combined with antibiotics against Staphylococcus aureus . The phage-antibiotic synergy (PAS) effect was evaluated using the fractional inhibitory concentration (FIC) and flow cytometric analysis. The determined minimum...

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Bibliographic Details
Published in:Food science and biotechnology 2016, 25(3), , pp.935-940
Main Authors: Jo, Ara, Ding, Tian, Ahn, Juhee
Format: Article
Language:English
Subjects:
antibiotic resistance
antimicrobial properties
bacteria
bacteriophages
cefoxitin
Chemistry
Chemistry and Materials Science
chloramphenicol
ciprofloxacin
flow cytometry
Food Science
minimum inhibitory concentration
Nutrition
polymyxin B
Staphylococcus aureus
식품과학
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Summary:This study was designed to assess the synergistic antimicrobial effect of phages combined with antibiotics against Staphylococcus aureus . The phage-antibiotic synergy (PAS) effect was evaluated using the fractional inhibitory concentration (FIC) and flow cytometric analysis. The determined minimum inhibitory concentration (MIC) values varied from 0.125 to 128 μg/mL for S. aureus KACC 13236 (SA S ) and from 0.25 to >256 μg/mL for S. aureus CCARM 3080 (SA R ). The PAS effect was more pronounced in SA S treated with phage SA11 in the presence of cefoxitin (FIC=0.62), chloramphenicol (FIC=0.54), and polymyxin B (FIC=0.38). SA S and SA R cells were injured when exposed to asublethal concentration of ciprofloxacin, whereas these cells were highly susceptible to the phage-antibiotic combined treatment, showing 96% of relative percentages of injured and dead cells. The results suggest that the combined treatment of phages and antibiotics can be used to improve antimicrobial efficacy against antibiotic-resistant bacteria.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-016-0153-0