Nrf2-mediated induction of phase 2 detoxifying enzymes by curled dock (Rumex crispus L.) seed extract

• Published in: Food science and biotechnology 2013, 22(3), , pp.795-802
• Main Authors: Kim, Hyo Jung, Jung, Chae Lim, Park, In Sil, Suh, Hwa-Jin, Kwon, Oh Oun, Kim, Jong-Sang
• Format: Article
• Language: English
• Subjects: Chemistry; Chemistry and Materials Science; Food Science; Nutrition; Research Article; Rumex crispus; 식품과학
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-013-0147-0

Curled dock ( Rumex crispus L.) is known to have several health benefits including antioxidant, antifungal, and anti-inflammatory effects. The potential to induce phase 2 detoxifying enzymes of butanol extract of curled dock (BECD) seeds was investigated. BECD increased the activity of NAD(P)H:quinone oxidoreductase and expression of phase 2 detoxifying enzymes such as heme oxygenase 1, Γ-glutamylcysteine synthetase, NAD(P)H:quinone oxidoreductase, and glutathione reductase, in both hepa1c1c7 and its mutant BPRc1 cells. The translocation of transcription factor nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) and binding to antioxidant response element (ARE) in the promoter region of phase 2 detoxifying/antioxidant enzymes were promoted by BECD in HepG2-C8 cells. Furthermore, BECD enhanced phosphorylation of Akt in hepatoma cell lines while Nrf2 activation induced by BECD was suppressed by an inhibitor of Akt. This result suggests that BECD induces phase 2 detoxifying enzymes through the Akt/Nrf2/ARE signaling pathway. BECD merits further study to be developed as detoxification agent.