Anti-obesity effect of grape skin extract in 3T3-L1 adipocytes

• Published in: Food science and biotechnology 2011, 20(3), , pp.635-642
• Main Authors: Jeong, Y.S., Bio Industry Center, Daegu Technopark, Daegu, Republic of Korea, Jung, H.K., Bio Industry Center, Daegu Technopark, Daegu, Republic of Korea, Cho, K.H., Yeungnam University, Gyeongsan, Republic of Korea, Youn, K.S., Catholic University of Daegu, Daegu, Republic of Korea, Hong, J.H., Catholic University of Daegu, Daegu, Republic of Korea
• Format: Article
• Language: English
• Subjects: 3T3-L1; adipocyte; anti-obesity; Chemistry; Chemistry and Materials Science; Food Science; grape skin extract; GRAPES; Nutrition; RAISIN; real time-PCR; Research Article; UVA; Vitaceae; 식품과학
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-011-0090-x

The aim of the present study was to determine the effects of grape skin which was well known as natural antioxidant using 3T3-L1 adipocyte, representative cells with morphological and biochemical characteristics of adipocytes. Grape skin extract (GSE) suppressed the differentiation of 3T3-L1 adipocytes, and decreased the triglycerides content with lipid accumulation. The adipogenesis inhibitory effect of GSE was confirmed in adipocyte hormone secretion, such as leptin and adiponectin, as well as glycerol-3-phosphate dehydrogenase activity. However, apoptosis of both mature pre-adipocytes and adipocytes was not induced by GSE. The level of gene and protein expression was observed to understand the antiobesity mechanism underlying GSE. GSE treatment of 3T3-L1 cells resulted in decrease in the adipogenic transcription factor (PPARγ, C/EBPα, and SREBP1) gene and protein expression, and preadipocyte secreted factor-1 mRNA was up-regulated compared to the adipocyte control. The lipolysis-related mRNA level (hormone-sensitive lipase, lipoprotein lipase, and perilipin) in adipocytes with the addition of grape skin was similar to preadipocytes. These results support the potential application of GSE as a natural anti-obesity material in the food industry.