Disinfection Effect and Its Mechanism of Electrolyzed Oxidizing Water on Spores of Bacillus subtilis var. niger

A study was carried out on the disinfection efficiency of electrolyzed oxidizing water (EOW) on spores of Bacillus subtilis var. niger. The results showed a remarkable fungicidal rate of 100% after 20 min duration of 191 mg/L active available chlorine (ACC). The disinfection effect was improved with...

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Bibliographic Details
Published in:Food science and biotechnology 2011, 20(4), , pp.889-895
Main Authors: Tang, Wenwei, Tongji University, Shanghai, China, Zeng, Xinping, Tongji University, Shanghai, China, Zhao, Yusheng, Tongji University, Shanghai, China, Ye, Guoqing, Tongji University, Shanghai, China, Gui, Wenchi, Tongji University, Shanghai, China, Ni, Yaming, Tongji University, Shanghai, China
Format: Article
Language:English
Subjects:
BACILLUS SUBTILIS
Cell walls
Chemistry
Chemistry and Materials Science
Chlorine
Damage
Disinfection
disinfection efficiency
disinfection factor
disinfection mechanism
DNA damage
electrolyzed oxidizing water
Food Science
Fungicides
Image transmission
Membrane permeability
Membranes
Nutrition
Oxidation
Research Article
spore of Bacillus subtilis var. niger
Spores
식품과학
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Summary:A study was carried out on the disinfection efficiency of electrolyzed oxidizing water (EOW) on spores of Bacillus subtilis var. niger. The results showed a remarkable fungicidal rate of 100% after 20 min duration of 191 mg/L active available chlorine (ACC). The disinfection effect was improved with increased ACC or prolonged disinfection time, while organic interferents exerted a strong concentration-dependent inhibition against the disinfection. The disinfection mechanism was also investigated at bio-molecular level. EOW decreased dehydrogenase activity, intensified membrane permeability, elevated suspension conductivity, and caused leakage of intracellular K+, proteins, and DNA, indicating a damage of cell walls and membranes. Effects of EOW on microbiological ultra-structures were also verified by transmission electronic microscopy (TEM) images, showing that EOW destroyed protective barriers of the microbe and imposed some damages upon the nucleus area.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-011-0123-5