Comparison of ELISA and HPLC methods for the determination of biogenic amines in commercial doenjang and gochujang

In order to conduct fast detection of biogenic amines (BAs) in doenjang and gochujang, a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine was compared with HPLC analysis. The histamine contents were determined from 0.7 to 420 mg/kg by CD-ELISA and 2.2 to 382.4 mg/kg by H...

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Published in:Food science and biotechnology 2011, 20(6), , pp.1747-1750
Main Authors: Kim, T.K., Korea University, Jochiwon, Republic of Korea, Lee, J.I., Korea University, Jochiwon, Republic of Korea, Kim, J.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Mah, J.H., Korea University, Jochiwon, Republic of Korea, Hwang, H.J., Korea University, Jochiwon, Republic of Korea, Kim, Y.W., Korea University, Jochiwon, Republic of Korea
Format: Article
Language:English
Subjects:
biogenic amine
Chemistry
Chemistry and Materials Science
competitive direct enzyme-linked immunosorbent assay
doenjang
Food Science
gochujang
HPLC
Indexing in process
Nutrition
Research Note
식품과학
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Summary:In order to conduct fast detection of biogenic amines (BAs) in doenjang and gochujang, a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine was compared with HPLC analysis. The histamine contents were determined from 0.7 to 420 mg/kg by CD-ELISA and 2.2 to 382.4 mg/kg by HPLC. Both methods showed a good agreement for histamine analysis (R=0.969). In addition, the histamine contents by CD-ELISA were correlated to the sum of 7 major BAs in the samples (R=0.910), suggesting that the CD-ELISA can be used as a rapid indicator for total BAs in doenjang and gochujang.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-011-0241-0