Detection of Lymphocystis Disease Virus (LCDV) in Olive Flounder (Paralichthys olivaceus) Using Efficient Extraction and Concentration Methods

• Published in: Food science and biotechnology 2010, 19(6), , pp.1693-1696
• Main Authors: Kim, D.W., Chonnam National University, Gwangju, Republic of Korea, Jeong, B.M., Chonnam National University, Yeosu, Republic of Korea, Kim, W.S., Chonnam National University, Yeosu, Republic of Korea, Kim, J.O., Chonnam National University, Yeosu, Republic of Korea, Lim, C.H., Sunchon National University, Sunchon, Republic of Korea, Cho, J.Y., Chonnam National University, Gwangju, Republic of Korea, Eun, J.B., Chonnam National University, Gwangju, Republic of Korea, Moon, J.H., Chonnam National University, Gwangju, Republic of Korea, Kim, S.R., National Fisheries Research and Development Institute, Busan, Republic of Korea, Park, M.A., National Fisheries Research and Development Institute, Busan, Republic of Korea, Oh, M.J., Chonnam National University, Yeosu, Republic of Korea
• Format: Article
• Language: English
• Subjects: Chemistry; Chemistry and Materials Science; fish; Food Science; Lymphocystis disease virus; Nutrition; PARALICHTHYS OLIVACEUS; PCR; Pleuronectiformes; Research Note; 식품과학
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-010-0241-5

Efficiency of extraction and concentration methods for the detection of the major capsid protein gene of lymphocystis disease virus from different tissues of olive flounders (Paralichthys olivaceus) was tested. Tris elution buffer showed a 100 fold higher polymerase chain reaction (PCR) detection limit than TE elution buffer in the virus extraction step from skin tissues. Using the TRPD (Tris elution buffer, polyethylene, and DNA extraction kit) procedure, we confirmed that skin tissues and lymphocystis cells of olive flounders had a detection limit of 10∨-6 and 10∨-7 PCR-U/μL, meaning that 10∨6 and 10∨7 fold dilutions of lymphocystis disease virus (LCDV) were PCR positive, respectively.