Rapid and Simple Biochemical Detection for Salmonella spp. Using Modified LB Broth and the MUCAP Test

In this study, we developed a rapid Salmonella detection system and confirmed its detection efficiency using modified selective pre-enrichment and the 4-methyl umbelliferyl caprilate (MUCAP) test. Luria-Bertani (LB) medium without auto-fluorescence was modified by the addition of a bile salt mixture...

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Bibliographic Details
Published in:Food science and biotechnology 2011, 20(1), , pp.201-207
Main Authors: Jang, K.I., Chungbuk National University, Cheongju, Republic of Korea, Ahn, J.B., Seowon University, Cheongju, Republic of Korea, Kim, K.Y., Chungbuk National University, Cheongju, Republic of Korea
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Enterobacter
Escherichia coli
Food Science
Nutrition
rapid detection
Research Article
SALMONELLA
Salmonella enteritidis
Staphylococcus aureus
식품과학
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Summary:In this study, we developed a rapid Salmonella detection system and confirmed its detection efficiency using modified selective pre-enrichment and the 4-methyl umbelliferyl caprilate (MUCAP) test. Luria-Bertani (LB) medium without auto-fluorescence was modified by the addition of a bile salt mixture and sodium citrate as inhibitors, after which ferrous sulfate and sodium thiosulfate were added for the induction of H₂S gas. In a mixed culture of Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis in Salmonella detective modified LB (SDMLB) medium, S. enteritidis dominated regardless of their initial population size. Among 10 bacterial strains, Salmonella and Enterobacter spp. were cultured and distinguished by the H₂S test because the Enterobacter spp. was unable to produce H₂S gas in SDMLB medium. MUCAP testing produced blue fluorescence only for Salmonella spp. Tests for Salmonella using chicken skin and egg shells revealed the presence of Salmonella spp. Thus, foods carrying Salmonellae were detected rapidly and simply using this method.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-011-0027-4