Linarin down-regulates phagocytosis, pro-inflammatory cytokine production, and activation marker expression in RAW264.7 macrophages

Plant-extracted flavonoid glycosides have been reported to be bioactive compounds with pleiotropic functions, including antioxidant, anti-inflammatory, and anti-cancer effects. This study investigated the anti-inflammatory role of linarin (acacetin-7-rutinoside, which is found in Chrysanthemum indic...

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Bibliographic Details
Published in:Food science and biotechnology 2016, 25(5), , pp.1437-1442
Main Authors: Kim, Bomi, Lee, Jong Hun, Seo, Myung-Ji, Eom, Seok Hyun, Kim, Wooki
Format: Article
Language:English
Subjects:
Anticancer properties
Antigen presentation
Antigens
Antioxidants
Beads
Bioactive compounds
Biocompatibility
CD80 antigen
Chemistry
Chemistry and Materials Science
Chrysanthemum
Cytokines
Cytotoxicity
Dendranthema
Flavone glycosides
Flavonoids
Fluorescence
Food Science
Glycosides
IL-1β
Inflammation
Interleukin 6
Lipopolysaccharides
Macrophages
Markers
Nitric oxide
Nutrition
Phagocytes
Phagocytosis
Plant extracts
Surface markers
Toxicity
식품과학
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Summary:Plant-extracted flavonoid glycosides have been reported to be bioactive compounds with pleiotropic functions, including antioxidant, anti-inflammatory, and anti-cancer effects. This study investigated the anti-inflammatory role of linarin (acacetin-7-rutinoside, which is found in Chrysanthemum indicum (Gam-Guk) and Dendranthema zawadskii (Gu-Jul-Cho)), on lipopolysaccharide-stimulated RAW264.7 macrophages. Linarin treatments exhibited no cytotoxicity up to a concentration of 30 μM, as assessed by MTT assay. The production of nitric oxide, an inflammatory mediator, was decreased by addition of linarin. The secretion of pro-inflammatory cytokines, interleukin-1β and interleukin-6, was significantly decreased in a dose-dependent manner. Linarin also decreased the phagocytic ability of macrophages following co-culture with fluorescent beads. In addition, expression levels of antigenpresenting surface markers, MHC II and CD80, were suppressed by linarin. Taken together, these results indicate that the flavonoid glycoside linarin has an anti-inflammatory effect, in part through the suppression of phagocytosis, cytokine production, and antigen presentation in macrophages.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-016-0223-3