Construction of a recombinant Escherichia coli JM109/A-68 for production of carboxymethylcellulase and comparison of its production with its wild type, Bacillus velezensis A-68 in a pilot-scale bioreactor

A gene encoding carboxymethylcellulase (CMCase) of Bacillus velezensis A-68 had been cloned in Escherichia coli JM109. Based on productivity and economic aspect, rice bran and ammonium chloride were chosen to be optimal carbon and nitrogen sources for production of CMCase by E. coli JM109/A-68. The...

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Published in:Biotechnology and bioprocess engineering 2016, 21(5), , pp.601-611
Main Authors: Kim, Myung-Hwan, Kang, Duk-Un, Lee, Jin-Woo
Format: Article
Language:English
Subjects:
Ammonium
Bacillus
Bacteria
Bioreactors
Biotechnology
Cell growth
Cells
Cellulase
Chemistry
Chemistry and Materials Science
Cloning
Design
E coli
Enzymes
Escherichia coli
Genes
Glucose
Industrial and Production Engineering
Nitrogen sources
Productivity
Research Paper
Rice
Rice bran
Sodium chloride
Statistical analysis
Variables
Variance analysis
생물공학
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creator Kim, Myung-Hwan
Kang, Duk-Un
Lee, Jin-Woo
description A gene encoding carboxymethylcellulase (CMCase) of Bacillus velezensis A-68 had been cloned in Escherichia coli JM109. Based on productivity and economic aspect, rice bran and ammonium chloride were chosen to be optimal carbon and nitrogen sources for production of CMCase by E. coli JM109/A-68. The optimal conditions for rice bran, ammonium chloride, and initial pH of medium for production of CMCase were established by the response surface methodology (RSM). The concentrations of four salts in the medium, K 2 HPO 4 , NaCl, MgSO 4 ·7H 2 O, and (NH 4 ) 2 SO 4 , for production of CMCase also were optimized. The optimal temperatures for cell growth and production of CMCase were 37°C. The maximal production of CMCase by E. coli JM109/A-68 was 880.2 U/mL, which was 10.5 time higher than its wild type, B. velezensis A-68. The production of CMCase by E. coli JM109/A-68 was compared with that by B. velezensis A-68 in a 100 L pilot-scale bioreactor under the optimized conditions. The production of CMCase by E. coli JM109/A-68 was found to be the mixed-growth associated unlike the growthassociated production of CMCase by B. velezensis A-68.
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source ABI/INFORM Global; Springer Link
subjects Ammonium
Bacillus
Bacteria
Bioreactors
Biotechnology
Cell growth
Cells
Cellulase
Chemistry
Chemistry and Materials Science
Cloning
Design
E coli
Enzymes
Escherichia coli
Genes
Glucose
Industrial and Production Engineering
Nitrogen sources
Productivity
Research Paper
Rice
Rice bran
Sodium chloride
Statistical analysis
Variables
Variance analysis
생물공학
title Construction of a recombinant Escherichia coli JM109/A-68 for production of carboxymethylcellulase and comparison of its production with its wild type, Bacillus velezensis A-68 in a pilot-scale bioreactor
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