Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin

Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyz...

Full description

Saved in:
Bibliographic Details
Published in:Journal of veterinary science (Suwŏn-si, Korea) 2016, 17(4), , pp.459-465
Main Authors: Alvarez, Rafael Herrera, Natal, Fabio Luis Nogueira, Ribela, Maria Teresa Carvalho Pinto, de Almeida, Beatriz Elane, de Oliveira, João Ezequiel, Bartolini, Paolo
Format: Article
Language:English
Subjects:
Animals
Chorionic Gonadotropin - analysis
Chromatography, High Pressure Liquid - veterinary
Chromatography, Reverse-Phase - veterinary
Female
Horses - physiology
Original
수의학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in comparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained by this qualitative and quantitative physical-chemical analysis were compared using an bioassay based on the ovarian weight gain of prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (t = 27.9 min), which were related to the bioactivity. Commercial preparations that have this altered peak showed very little or no activity, as demonstrated by rat ovarian weight and in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changes in the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.
ISSN:1229-845X
1976-555X
DOI:10.4142/jvs.2016.17.4.459