The Influences of Different Ratios of Biphasic Calcium Phosphate and Collagen Augmentation on Posterior Lumbar Spinal Fusion in Rat Model

• Published in: Yonsei medical journal 2017, 58(2), , pp.407-414
• Main Authors: Kim, Kyung Hyun, Park, Jeong Yoon, Park, Hyo Suk, Kim, Keun Su, Chin, Dong Kyu, Cho, Yong Eun, Kuh, Sung Uk
• Format: Article
• Language: English
• Subjects: Animals; Bone Transplantation; Collagen - administration & dosage; Hydroxyapatites - administration & dosage; Original; Palpation; Prostheses and Implants; Rats; Spinal Fusion - methods; Transplantation, Autologous; X-Ray Microtomography; 의학일반
• ISSN: 0513-5796; 1976-2437
• DOI: 10.3349/ymj.2017.58.2.407

To determine the influence of different ratios of hydroxyapatite (HA)/beta tricalcium phosphate (β-TCP) and collagen augmentation for posterior lumbar fusion in a rat model. We generated a posterior lumbar fusion model in 50 rats and divided it into five groups of equal number as follows; 1) autologous bone graft as group A, 2) 70% HA+30% β-TCP as group B, 3) 70% HA+30% β-TCP+collagen as group C, 4) 30% HA+70% β-TCP as group D, and 5) 30% HA+70% β-TCP+collagen as group E. Rats were euthanized at 12 weeks after surgery and fusion was assessed by manual palpation, quantitative analysis using microCT and histology. The score of manual palpation was significantly higher in group C than group E (3.1±1.1 vs. 1.8±0.8, p=0.033). However, in terms of microCT analysis, group D showed significantly higher scores than group B (5.5±0.8 vs. 3.1±1.1, p=0.021). According to quantitative volumetric analysis, 30% HA+70% β-TCP groups (group D and E) showed significantly reduced fusion mass at 12 weeks after surgery (123±14.2, 117±46.3 vs. 151±27.3, p=0.008, 0.003, respectively). Collagen augmentation groups revealed superior results in terms of both microCT score and histologic grade. A 7:3 HA/β-TCP ratio with collagen augmentation rather than a 3:7 HA/β-TCP ratio could be a more favorable graft substitute for lumbar spinal fusion. There was positive role of collagen as an adjunct for spinal bone fusion process.