Novel phenotypes of Drosophila spinster locus on the head formation during embryogenesis

spinster ( spin ) is a late endosome/lysosome membrane protein with the amino acid sequence of a lysosomal sugar carrier and expressed in the glial cells. Spin is required for autophagy and lysosome reformation by releasing lysosomal degradation products of autolysosome into the cytosol in Drosophil...

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Bibliographic Details
Published in:Genes & genomics 2017, 39(2), , pp.237-242
Main Authors: Kim, Ji-Sun, Park, Ki-Seok, Park, Ok-kyu, Kim, Sang Hee, Jeon, Sang-Hak
Format: Article
Language:English
Subjects:
Amino acid sequence
Animal Genetics and Genomics
Autophagy
Biomedical and Life Sciences
Cytosol
Degradation products
Embryogenesis
Glial cells
Human Genetics
Insects
Life Sciences
Lysosomes
Membrane proteins
Microbial Genetics and Genomics
Phagocytosis
Plant Genetics and Genomics
Protein turnover
Research Article
Sugar
생물학
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Summary:spinster ( spin ) is a late endosome/lysosome membrane protein with the amino acid sequence of a lysosomal sugar carrier and expressed in the glial cells. Spin is required for autophagy and lysosome reformation by releasing lysosomal degradation products of autolysosome into the cytosol in Drosophila larvae and adults. However, such kind of function has not been investigated in embryos yet. In this study, for the first time, we examined the effects of spin mutation on the endocytic pathway and autophagy during embryogenesis. Loss-of-function spin mutation led to the abnormal process of early endosome/recycling endosome and the accumulation of enlarged autophagosome/autolysosome. These abnormal endocytic pathway and autophagy subsequently caused the malformation of head at embryonic stages. These results show that Spin is involved in the endocytic pathway and autophagy during embryogenesis as well as larval and adult stages.
ISSN:1976-9571
2092-9293
DOI:10.1007/s13258-016-0513-4