Evaluation of zinc finger E-box binding homeobox 1 and transforming growth factor-beta2 expression in bladder cancer tissue in comparison with healthy adjacent tissue

• Published in: Investigative and clinical urology 2017, 58(2), , pp.140-145
• Main Authors: Mahdavinezhad, Ali, Yadegarazari, Reza, Mousavi-Bahar, Seyed Habibollah, Poorolajal, Jalal, Jafari, Mohammad, Amirzargar, Mohammad Ali, Effatpanah, Hosein, Saidijam, Massoud
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Case-Control Studies; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Middle Aged; Neoplasm Grading; Neoplasm grading; Transforming growth factor beta2; Urinary bladder neoplasms; ZEB1 protein; Neoplasm Invasiveness; Original; RNA, Messenger - genetics; RNA, Neoplasm - genetics; Specimen Handling - methods; Transforming Growth Factor beta2 - biosynthesis; Transforming Growth Factor beta2 - genetics; Up-Regulation; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; Zinc Finger E-box-Binding Homeobox 1 - biosynthesis; Zinc Finger E-box-Binding Homeobox 1 - genetics; 비뇨기과학
• ISSN: 2466-0493; 2466-054X
• DOI: 10.4111/icu.2017.58.2.140

The fifth most common cancer is allocated to bladder cancer (BC) worldwide. Understanding the molecular mechanisms of BC invasion and metastasis to identify target therapeutic strategies will improve disease survival. So the aim of this study was to measure expression rate of zinc finger E-box binding homeobox 1 (ZEB1) and transforming growth factor-beta2 (TGF-β2) mRNA in tissue samples of patients with BC and its healthy adjacent tissue samples and their association with muscle invasion, size and grade of the tumor. Tissue samples were collected from 35 newly diagnosed untreated patients with BC from 2013 to 2014. Total RNA was extracted from about 50-mg tissue samples using TRIzol reagent. TAKARA SYBR Premix EX Tag II was applied to determine the rate of mRNA expression by real-time polymerase chain reaction (PCR). To obtain final validation, PCR product of ZEB1 and TGF-β2 were sequenced. STATA 11 software was used to analyze the data. The expression level of ZEB1 in tumor samples was significantly more than of in healthy adjacent tissue samples. Up-regulation of TGF-β2 showed a strong association with muscle invasion (p=0.017). There was also demonstrated a relationship between over expression of ZEB1 with the tumor size (p=0.050). It looks ZEB1 and TGF-β2 had a role in BC patients. In this study ZEB1 expression was higher in BC tissues than that of in healthy control tissues. There was demonstrated a markedly association between overexpression of TGF-β2 and muscle invasion. Therefore, they are supposed to be candidate as potential biomarkers for early detection and progression of BC.