The effects of pentoxifylline adminstration on fracture healing in a postmenopausal osteoporotic rat model

• Published in: Laboratory animal research 2017, 33(1), , pp.15-23
• Main Authors: Vashghani Farahani, Mohammad Mahdi, Ahadi, Reza, Abdollahifar, Mohammadamin, Bayat, Mohammad
• Format: Article
• Language: English
• Subjects: femur; hounsfield unit biomechanical phenomena; Original; Osteoporosis; osteotomy; ovariectomy; rat; 수의학
• ISSN: 1738-6055; 2233-7660
• DOI: 10.5625/lar.2017.33.1.15

Previous studies report positive effects of pentoxifylline (PTX) alone or in combination with other drugs on some pathologic bone diseases as well as an ability to accelerate osteogensis and fracture healing in both animal models and human patients. The aim of this present study was to evaluate the effects of PTX administration on Hounsfield unit and bone strength at catabolic response (bone resorbing) of a fracture in an experimental rat model of ovariectomy induced osteoporosis (OVX-D). Thirty adult female rats were divided into groups as follows: 1 (OVX, control, no treatment); 2 (OVX, sham: daily distilled water); 3 (OVX, daily alendronate: 3 mg/kg); 4 (OVX, twice daily 100 mg/kg PTX) and 5 (OVX, PTX+alenderonate). OVX was induced by bilateral ovariectomy in all rats. A complete standardized osteotomy of the right femur was made after 3.5 months. PTX and alendronate treatments were performed for eight weeks. Then, rats were euthanized and had its right femur subjected to computerized tomography scanning for measuring Hounsfield unit; eventually, the samples were sent for a three point bending test for evaluation of the bone strength. Administration of PTX with 200 mg/kg and alendronate alone and in combination showed no significant alteration in Hounsfield unit and biomechanical properties of repairing callus of the complete osteotomy compared with the control group. Results showed increased bending stiffness and stress high load mean values of repairing complete osteotomy in PTX-treated rats compared to the control OVX-D.