Overexpression of Nuclear Factor Y subunit StNF-YA7 enhances drought tolerance in potato

Drought, the most devastating abiotic stress, has detrimental effects on crop production. To minimize such adverse effects, it is crucial to improve drought tolerance in crops. Several members of the plant Nuclear Factor Y (NF-Y) gene family are involved in drought tolerance. In this study, we isola...

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Published in:Horticulture, environment and biotechnology 2017, Environment, and Biotechnology, 58(2), , pp.170-177
Main Authors: Na, Jong-Kuk, Kim, Kyung Hwa, Seong, Eun-Soo, Kim, Beom-Gi, Cho, Kwang Soo, Cho, Ji Hong, Park, Soo-Kwon, Kim, Dool-Yi
Format: Article
Language:English
Subjects:
Abiotic stress
Abscisic acid
Agriculture
Assaying
Biomedical and Life Sciences
Crop production
Drought
Drought resistance
Leaves
Life Sciences
Nuclei
Plant Breeding/Biotechnology
Plant Ecology
Plant Physiology
Plant tissues
Potatoes
Research Report
Side effects
Solanum tuberosum
Stresses
Transgenic plants
Vascular system (plant anatomy)
Vascular tissue
Vegetables
Water loss
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Summary:Drought, the most devastating abiotic stress, has detrimental effects on crop production. To minimize such adverse effects, it is crucial to improve drought tolerance in crops. Several members of the plant Nuclear Factor Y (NF-Y) gene family are involved in drought tolerance. In this study, we isolated the potato NF-Y subunit A 7 ( StNF-YA7 ) gene from Solanum tuberosum ‘Superior’ and analyzed its relationship to abiotic stress tolerance. StNF-YA7 was induced by drought, salt stress, and abscisic acid (ABA), and a transient expression assay using 35S:StNF-YA7-smGFP showed that StNF-YA7 is localized to the nucleus, as reported for other plant NF-Ys. To examine the involvement of StNF-YA7 in drought tolerance, we generated 35S:StNF-YA7 transgenic potato plants. The transgenic potato plants exhibited enhanced drought tolerance, likely due to reduced leaf water loss. Analysis of the StNF-YA7 promoter ( pStNF-YA7 ) fused to GUS revealed that StNF-YA7 is strongly expressed in vascular tissue. Taken together, these results suggest that enhancing drought tolerance via StNF-YA7 overexpression is associated with reduced leaf water loss, likely through mechanism(s) involving the vascular system.
ISSN:2211-3452
2211-3460
DOI:10.1007/s13580-017-0200-7