Limnobacter humi sp. nov., a thiosulfate-oxidizing, heterotrophic bacterium isolated from humus soil, and emended description of the genus Limnobacter Spring et al. 2001

• Published in: The journal of microbiology 2017, 55(7), , pp.508-513
• Main Authors: Nguyen, Tuan Manh, Kim, Jaisoo
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Burkholderiaceae - classification; Burkholderiaceae - genetics; Burkholderiaceae - isolation & purification; Burkholderiaceae - metabolism; Cardiolipin; Decomposing organic matter; Deoxyribonucleic acid; DNA; DNA, Bacterial; DNA, Ribosomal; Fatty acids; Fatty Acids - analysis; Gene sequencing; Heterotrophic Processes; Humus; Hybridization; Life Sciences; Lipids; Microbial Systematics and Evolutionary; Microbiological strains; Microbiology; New species; Nucleic Acid Hybridization; Nucleotide sequence; Oxidation; Oxidation rate; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - analysis; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sequencing; Similarity; Soil; Soil Microbiology; Spring; Thiosulfate; Thiosulfates - metabolism; Ubiquinone; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-017-6645-7

Three Gram-negative, strictly aerobic, chemolithoheterotrophic bacterial strains, designated UCM-30, UCM-33, and UCM-39 T , were isolated in South Korea. Based on their 16S rRNA gene sequences, the three isolated strains were found to be similar to Limnobacter thiooxidans CS-K2 T (97.41–97.68%), Limnobacter litoralis KP1-19 T (95.55–95.76%), and various genera belonging to the class Betaproteobacteria (90.34–93.34%). DNA-DNA hybridization showed 79.3–83.9% similarity between the genomic DNA of UCM-39 T , UCM-30, and UCM-33, while the sequence similarity between UCM-39 T and L. thiooxidans KACC 13837T or L. litoralis LMG 24869T was 23.7% and 18.6%, respectively. The DNA G+C content of UCM 39T was 59.7 mol%, the major ubiquinone was Q-8, and the optimal oxidation rate was observed at 10 mM thiosulfate. The major fatty acids (≥ 10%) were summed features 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ) and 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ), and C 16:0 . The major polar lipids (diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol) were found in all members of genus Limnobacter . Based on phenotypic, physiological, and phylogenetic analyses, the UCM-39T strain was found to be significantly distinct to represent a novel species affiliated to the genus Limnobacter . We propose to name it Limnobacter humi sp. nov. with the type strain UCM-39 T (=KACC 18574 T =NBRC 111650 T ).