Response surface methodology for the evaluation of guanidine hydrochloride partitioning in polymer-salt aqueous two-phase system

The current study employed response surface methodology (RSM) with a face-centered central composite design (CCD) to indicate the essential variables on the partition coefficient of guanidine hydrochloride (GuHCl) in the poly (ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS). To eval...

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Bibliographic Details
Published in:The Korean journal of chemical engineering 2017, 34(7), 208, pp.2033-2042
Main Authors: Pirdashti, Mohsen, Movagharnejad, Kamyar, Rostami, Abbas Ali, Shahrokhi, Behnia
Format: Article
Language:English
Subjects:
Binary systems
Biotechnology
Catalysis
Chemistry
Chemistry and Materials Science
Ethylene glycol
Guanidine hydrochloride
Independent variables
Industrial Chemistry/Chemical Engineering
Materials Science
Mathematical models
Molecular weight
Partitioning
Potassium
Response surface methodology
Separation Technology
Thermodynamics
화학공학
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Summary:The current study employed response surface methodology (RSM) with a face-centered central composite design (CCD) to indicate the essential variables on the partition coefficient of guanidine hydrochloride (GuHCl) in the poly (ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS). To evaluate the partition coefficients of GuHCl in the mentioned ATPS, the pH (7.0, 8.5 and 10.0), GuHCl concentration (1.0, 3.5 and 6.0% w/w), PEG molecular weight (2,000, 4,000 and 6,000 gmol −1 ) and PEG/potassium phosphate concentrations ratio were selected as independent variables. A quadratic model is suggested to find the impact of these variables. The suggested model has a strong harmony with the experimental data. The results of the model display that the GuHCl concentration and weight percent of the salt in feed have a large and small influence on the GuHCl partitioning.
ISSN:0256-1115
1975-7220
DOI:10.1007/s11814-017-0108-z