Diverse molecular resistance mechanisms of Bacillus megaterium during metal removal present in a spent catalyst

• Published in: Biotechnology and bioprocess engineering 2017, 22(3), , pp.296-307
• Main Authors: Rivas-Castillo, Andrea, Orona-Tamayo, Domancar, Gómez-Ramírez, Marlenne, Rojas-Avelizapa, Norma G.
• Format: Article
• Language: English
• Subjects: Aluminum; Bacteria; Bioremediation; Biotechnology; Catalysts; Chemistry; Chemistry and Materials Science; Heavy metals; Industrial and Production Engineering; Materials recovery; Metals; Microorganisms; Molecular modelling; Molybdenum; Nickel; Oxidative stress; Petrochemicals; Petrochemicals industry; Proteins; Research Paper; Strain; Vanadium; Wastes; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-016-0019-6

Bacillus megaterium strain MNSH1-9K-1, isolated from a high-metal content site in Guanajuato, Mexico, has the intrinsic capacity to remove vanadium (V) and nickel (Ni) from a petrochemical spent catalyst, and counteract the toxic effects produced in the cell due to the presence of oxidative stress. Since knowledge of the molecular components involved in the microbial resistance to spent catalysts is scarce, this study aimed to identify the proteins potentially involved in the enhanced resistance of a B. megaterium strain, during the removal of metals contained in a spent catalyst. Thus, the current research uses a proteomic approach to investigate and evidence the differences in the molecular resistance mechanisms of two B. megaterium strains, one isolated from a mining site and a wild type strain, when both are exposed to a spent catalyst. In addition, we studied their ability to eliminate nickel (Ni), vanadium (V), aluminum (Al) and molybdenum (Mo). The data presented here may contribute to the knowledge of the molecular mechanisms involved in the resistance of B. megaterium to high metal content wastes, as well as its potential utilization for the recovery of valuable industrial metals.