Assessment of the quantitative ability of AdvanSure TB/NTM real-time PCR in respiratory specimens by comparison with phenotypic methods

Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM real-time PCR kit (LG Life Science, Korea) to determine the cycle threshold (Ct) for m...

Full description

Saved in:
Bibliographic Details
Published in:Annals of laboratory medicine 2014, 34(1), , pp.51-55
Main Authors: Lee, Hyeyoung, Park, Kang Gyun, Lee, Gundong, Park, Joonhong, Park, Yong-Gyu, Park, Yeon-Joon
Format: Article
Language:English
Subjects:
Area Under Curve
Brief Communication
Bronchoalveolar Lavage Fluid - microbiology
DNA, Bacterial - analysis
Humans
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Phenotype
Reagent Kits, Diagnostic
Real-Time Polymerase Chain Reaction
Retrospective Studies
ROC Curve
Sensitivity and Specificity
Sputum - microbiology
Tuberculosis - diagnosis
Tuberculosis - microbiology
병리학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM real-time PCR kit (LG Life Science, Korea) to determine the cycle threshold (Ct) for mycobacterial burden. We retrospectively analyzed data from 108 patients whose respiratory specimens (sputums and bronchoalveolar lavage fluids) were positive for Mycobacterium tuberculosis complex (85 culture-positive and 23 culture-negative specimens). We compared Ct values with grades of acid-fast bacilli (AFB) staining, semi-quantitative colony count on solid medium, and time to positivity (TTP) in liquid and solid media. We also investigated the cutoff Ct value for predicting stain-positive status. Ct value showed significant reverse correlation with AFB staining grade (r s=-0.635, P
ISSN:2234-3806
2234-3814
DOI:10.3343/alm.2014.34.1.51