Effects of lignocellulose-derived inhibitors on growth and succinic acid accumulation by Corynebacterium glutamicum

• Published in: Biotechnology and bioprocess engineering 2015, 20(4), , pp.744-752
• Main Authors: Xu, Hong-Tao, Wang, Chen, Zhou, Zhi-Hui, Chen, Zhong-Jun, Cai, Heng
• Format: Article
• Language: English
• Subjects: Accumulation; Acid production; Aldehydes; Analysis; Bacteria; Biomass; Biotechnology; By products; Charcoal; Chemicals; Chemistry; Chemistry and Materials Science; corn cobs; Corynebacterium glutamicum; Engineering; Enzymatic activity; enzyme activity; Enzymes; Fermentation; Food; furfural; Glucose; growth retardation; hemicellulose; hydrolysates; Hydrolysis; hydroxymethylfurfural; Industrial and Production Engineering; Lignocellulose; malic enzyme; Organic acids; Organic chemicals; oxygen; Polymers; protocatechuic acid; Research Paper; Studies; succinic acid; syrups; Toxicity; vanillin; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-015-0201-2

Succinic acid production by genetically engineered C. glutamicum from lignocellulosic biomass requires the hydrolysis of carbohydrate polymers into fermentable syrup. A variety of toxic compounds are produced such as aldehydes and organic acids, while the hydrolysis of hemicellulose with dilute acid. In this study, we have investigated the toxicity of representative aldehydes (furfural, 5-hydroxymethylfurfural, syringaldehyde, and vanillin) and organic acids (ferulic, 4-hydroxybenzic, vanillic, protocatechuic acid) on growth and succinic acid accumulation of C. glutamicum NC-1. In the presence of various inhibitors of growth experiment, furfural, 5- hydroxymethylfurfural appeared less toxic to growth of C. glutamicum NC-1, syringaldehyde almost completely inhibitor growth of C. glutamicum NC-1, vanillin has inhibited the growth of 67%, of organic acids, only ferulic appeared toxic to growth of C. glutamicum NC-1. Of succinic acid accumulation experiment under oxygen deprivation, all the organic acids compounds showed little inhibition on the glocuse consumption and succinic acid accumulation of C. glutamicum NC-1, but furfural, 5- hydroxymethylfurfural and vanillic have decreased the production of succinic acid. In addition, the actual inhibitor mixtures from the acid hydrolysate of corn cobs have reduced the accumulation of succinic acid. Across further research showed, a reason of succinic acid yield decrease was the malic enzyme activity was inhibited.