Enrichment as a screening method for a high-growth-rate microalgal strain under continuous cultivation system

Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to scr...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2016, 21(2), , pp.268-273
Main Authors: Shin, Won-Sub, Lee, Hansol, Sung, Min-Gyu, Hwang, Kwon-Tack, Jung, Simon MoonGeun, Kwon, Jong-Hee
Format: Article
Language:English
Subjects:
Algae
Alternative energy sources
Analysis
Biodiesel fuels
Biofuels
Biomass
Biosynthesis
Biotechnology
Cell growth
Cellular biology
Chemistry
Chemistry and Materials Science
Chlamydomonas reinhardtii
Chlorella protothecoides
Chlorella vulgaris
Climate change
Cultivation
Industrial and Production Engineering
Lipids
Membrane reactors
Microalgae
Productivity
Raw materials
Research Paper
Sensors
Studies
Test organisms
생물공학
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Summary:Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to screen a high-growth-rate microalga from a mixed culture of microalgal species; Chlorella vulgaris, Chlorella protothecoides , and Chlamydomonas reinhardtii were used as test organisms for our experiments. The time-dependent washout of mixed microalgal pool was executed to successfully enrich the C. reinhardtii , which exhibits the higher growth rate than C. vulgaris and C. protothecoides under turbidostat conditions within 75 h. The domination of C. reinhardtii in the mixed culture was validated by on-line monitoring of growth rate and flowcytometric analysis. For the time-efficient production of microalgal biomass, this screening process has a high potential to segregate the fast-growing microalgal strains from the pool of various uncharacterized microalgal species and random mutants.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-015-0716-6