Isolation and characterization of collagen from marine fish (Thunnus obesus)

In the present study, we isolated collagen from Thunnus obesus bone, which was physiochemically characterized. Two different kinds of methods were used to isolate the collagen; they are the Acid Soluble Collagen (ASC) and Acid Soluble Enzyme Collagen (ASEC) methods. The isolated collagen was charact...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2013, 18(6), , pp.1185-1191
Main Authors: Jeong, Hee-Seok, Venkatesan, Jayachandran, Kim, Se-Kwon
Format: Article
Language:English
Subjects:
Acids
Animal skins
Arthritis
biocompatibility
Biomedical materials
Biotechnology
Bones
Cell growth
Chemistry
Chemistry and Materials Science
Collagen
Cosmetics
Cytotoxicity
Enzymes
Fish
Food
foods
Fourier transform infrared spectroscopy
Fourier transforms
Industrial and Production Engineering
industry
Infrared spectroscopy
leather
Light microscopy
Marine
Marine fish
Molecular weight
Motion picture industry
Natural products
Pharmaceuticals
polyacrylamide gel electrophoresis
Polypeptides
Proteins
Research Paper
Scanning electron microscopy
Studies
Thunnus obesus
Tissue engineering
Vertebrates
생물공학
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Summary:In the present study, we isolated collagen from Thunnus obesus bone, which was physiochemically characterized. Two different kinds of methods were used to isolate the collagen; they are the Acid Soluble Collagen (ASC) and Acid Soluble Enzyme Collagen (ASEC) methods. The isolated collagen was characterized with Fourier Transform Infrared Spectroscopy (FT-IR), SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Optical Microscopy (OM) and Scanning Electron Microscopy (SEM). FT-IR results revealed the presence of collagen. SEM and OM results depicted that collagen was in the form of fiber sponge-like scaffolds. The isolated collagen scaffold was checked with pre-osteoblast (MC3T3-E1) cell line for biocompatibility. The in vitro results revealed that the collagen scaffolds were highly biocompatible and nontoxic in nature. Herewith, we are suggesting that marine fish-derived collagen will be an excellent material for leather, film industry, pharmaceutical, cosmetics, biomedical and food applications.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-013-0316-2