Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli

In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to...

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Published in:Biotechnology and bioprocess engineering 2014, 19(2), , pp.327-331
Main Authors: Vo, Tam Dinh Le, Pham, Van Dung, Ko, Ji-seun, Lee, Seung Hwan, Park, Si Jae, Hong, Soon Ho
Format: Article
Language:English
Subjects:
Acid production
Analysis
Biopolymers
Biosynthesis
Biotechnology
butyric acid
Chemistry
Chemistry and Materials Science
Cloning
E coli
Engineering
Escherichia coli
gamma-aminobutyric acid
Gene expression
glutamate decarboxylase
Industrial and Production Engineering
Metabolism
monosodium glutamate
Plasmids
Pyrococcus horikoshii
Research Paper
Sodium
Studies
생물공학
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Summary:In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-013-0713-6