Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods

The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agi...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2008, 13(6), , pp.690-696
Main Authors: Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea), Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany), Beckmann, Martin (Technical University of Berlin, Berlin, Germany), Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea), Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr
Format: Article
Language:English
Subjects:
Biotechnology
brewing yeast
Chemistry
Chemistry and Materials Science
Chromatography
Cultivation
Fermentation
filter paper preservation
Industrial and Production Engineering
Metabolism
Methods
Nitrogen
Studies
TREHALOSA
TREHALOSE
VIABILIDAD
VIABILITE
VIABILITY
Yeast
Yeasts
생물공학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63
cites cdi_FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63
container_end_page 696
container_issue 6
container_start_page 690
container_title Biotechnology and bioprocess engineering
container_volume 13
creator Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea)
Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany)
Beckmann, Martin (Technical University of Berlin, Berlin, Germany)
Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea)
Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr
description The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agitation and aeration during cultivation had a positive effect regarding viability of the bottom-fermenting strains, Rh and Frank. For effective preservation, it was necessary to harvest yeast cells from the stationary phase during cultivation. For lyophilization preservation, the yeast strains tested showed a negative effect on viability, independent of strain or cultivation method. No significant correlation was found between trehalose concentration and yeast viability following either filter paper or lyophilization preservation. However, the filter paper preservation method was suitable for both bottom and top brewing yeast strains with regard to feasibility, viability, and maintenance of the yeast's specific character.
doi_str_mv 10.1007/s12257-008-0066-8
format article
fullrecord <record><control><sourceid>proquest_nrf_k</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_177218</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>21298616</sourcerecordid><originalsourceid>FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63</originalsourceid><addsrcrecordid>eNp9kcFu1DAQhiNEJUrhATggWRwQl7QeJ7GdY1l1oeoiqmpB4mR5k_Gu29Te2t6i5UH6vHWaSkgcONieGX3_L4_-ongH9BgoFScRGGtESanMh_NSvigOoRW8rCTwl7lmLA8rwV4Vr2O8prQWUsrD4uHMGOwS8YbMdkOy9zpZ78jMu96OVSS5Wwbc6MFHHOcJXSLa9eSn1Ss72LQfxZ8D_rZuTX6hjonM_TD4p_4yYMTw7HpvNZnbIWEgl3qbbx_IYu-3m2zzZ0K-Ydr4Pr4pDoweIr59fo-KH_Oz5exrufj-5Xx2uii7mkIqW1hJ1sq-Z11TN00lq1XXGypWLfTjfsBpJ1A3fUWlFsb0wJuWtxUYgZ1BXh0VnyZfF4y66azy2j69a69ugjq9Wp4rEIKBzOjHCd0Gf7fDmNStjR0Og3bod1ExyD_hMHp--Ae89rvg8hqKsbaGWvDRDSaoCz7GgEZtg73VYa-AqjFRNSWqcqJqTFSNGjZpYmbdGsNf4_-J3k8io73S62CjurhilLaUMgqsegQMNa3w</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>229414768</pqid></control><display><type>article</type><title>Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods</title><source>ABI/INFORM Global</source><source>Springer Link</source><creator>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea) ; Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany) ; Beckmann, Martin (Technical University of Berlin, Berlin, Germany) ; Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea) ; Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</creator><creatorcontrib>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea) ; Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany) ; Beckmann, Martin (Technical University of Berlin, Berlin, Germany) ; Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea) ; Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</creatorcontrib><description>The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agitation and aeration during cultivation had a positive effect regarding viability of the bottom-fermenting strains, Rh and Frank. For effective preservation, it was necessary to harvest yeast cells from the stationary phase during cultivation. For lyophilization preservation, the yeast strains tested showed a negative effect on viability, independent of strain or cultivation method. No significant correlation was found between trehalose concentration and yeast viability following either filter paper or lyophilization preservation. However, the filter paper preservation method was suitable for both bottom and top brewing yeast strains with regard to feasibility, viability, and maintenance of the yeast's specific character.</description><identifier>ISSN: 1226-8372</identifier><identifier>EISSN: 1976-3816</identifier><identifier>DOI: 10.1007/s12257-008-0066-8</identifier><language>eng</language><publisher>Heidelberg: The Korean Society for Biotechnology and Bioengineering</publisher><subject>Biotechnology ; brewing yeast ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Cultivation ; Fermentation ; filter paper preservation ; Industrial and Production Engineering ; Metabolism ; Methods ; Nitrogen ; Studies ; TREHALOSA ; TREHALOSE ; VIABILIDAD ; VIABILITE ; VIABILITY ; Yeast ; Yeasts ; 생물공학</subject><ispartof>Biotechnology and Bioprocess Engineering, 2008, 13(6), , pp.690-696</ispartof><rights>The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63</citedby><cites>FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/229414768/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/229414768?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,11669,27905,27906,36041,36042,44344,74644</link.rule.ids><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001300731$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany)</creatorcontrib><creatorcontrib>Beckmann, Martin (Technical University of Berlin, Berlin, Germany)</creatorcontrib><creatorcontrib>Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea)</creatorcontrib><creatorcontrib>Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</creatorcontrib><title>Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods</title><title>Biotechnology and bioprocess engineering</title><addtitle>Biotechnol Bioproc E</addtitle><description>The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agitation and aeration during cultivation had a positive effect regarding viability of the bottom-fermenting strains, Rh and Frank. For effective preservation, it was necessary to harvest yeast cells from the stationary phase during cultivation. For lyophilization preservation, the yeast strains tested showed a negative effect on viability, independent of strain or cultivation method. No significant correlation was found between trehalose concentration and yeast viability following either filter paper or lyophilization preservation. However, the filter paper preservation method was suitable for both bottom and top brewing yeast strains with regard to feasibility, viability, and maintenance of the yeast's specific character.</description><subject>Biotechnology</subject><subject>brewing yeast</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Cultivation</subject><subject>Fermentation</subject><subject>filter paper preservation</subject><subject>Industrial and Production Engineering</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Nitrogen</subject><subject>Studies</subject><subject>TREHALOSA</subject><subject>TREHALOSE</subject><subject>VIABILIDAD</subject><subject>VIABILITE</subject><subject>VIABILITY</subject><subject>Yeast</subject><subject>Yeasts</subject><subject>생물공학</subject><issn>1226-8372</issn><issn>1976-3816</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNp9kcFu1DAQhiNEJUrhATggWRwQl7QeJ7GdY1l1oeoiqmpB4mR5k_Gu29Te2t6i5UH6vHWaSkgcONieGX3_L4_-ongH9BgoFScRGGtESanMh_NSvigOoRW8rCTwl7lmLA8rwV4Vr2O8prQWUsrD4uHMGOwS8YbMdkOy9zpZ78jMu96OVSS5Wwbc6MFHHOcJXSLa9eSn1Ss72LQfxZ8D_rZuTX6hjonM_TD4p_4yYMTw7HpvNZnbIWEgl3qbbx_IYu-3m2zzZ0K-Ydr4Pr4pDoweIr59fo-KH_Oz5exrufj-5Xx2uii7mkIqW1hJ1sq-Z11TN00lq1XXGypWLfTjfsBpJ1A3fUWlFsb0wJuWtxUYgZ1BXh0VnyZfF4y66azy2j69a69ugjq9Wp4rEIKBzOjHCd0Gf7fDmNStjR0Og3bod1ExyD_hMHp--Ae89rvg8hqKsbaGWvDRDSaoCz7GgEZtg73VYa-AqjFRNSWqcqJqTFSNGjZpYmbdGsNf4_-J3k8io73S62CjurhilLaUMgqsegQMNa3w</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea)</creator><creator>Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany)</creator><creator>Beckmann, Martin (Technical University of Berlin, Berlin, Germany)</creator><creator>Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea)</creator><creator>Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</creator><general>The Korean Society for Biotechnology and Bioengineering</general><general>Springer Nature B.V</general><general>한국생물공학회</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>M7N</scope><scope>ACYCR</scope></search><sort><creationdate>20081201</creationdate><title>Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods</title><author>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea) ; Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany) ; Beckmann, Martin (Technical University of Berlin, Berlin, Germany) ; Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea) ; Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biotechnology</topic><topic>brewing yeast</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Cultivation</topic><topic>Fermentation</topic><topic>filter paper preservation</topic><topic>Industrial and Production Engineering</topic><topic>Metabolism</topic><topic>Methods</topic><topic>Nitrogen</topic><topic>Studies</topic><topic>TREHALOSA</topic><topic>TREHALOSE</topic><topic>VIABILIDAD</topic><topic>VIABILITE</topic><topic>VIABILITY</topic><topic>Yeast</topic><topic>Yeasts</topic><topic>생물공학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany)</creatorcontrib><creatorcontrib>Beckmann, Martin (Technical University of Berlin, Berlin, Germany)</creatorcontrib><creatorcontrib>Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea)</creatorcontrib><creatorcontrib>Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Korean Citation Index</collection><jtitle>Biotechnology and bioprocess engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea)</au><au>Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany)</au><au>Beckmann, Martin (Technical University of Berlin, Berlin, Germany)</au><au>Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea)</au><au>Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods</atitle><jtitle>Biotechnology and bioprocess engineering</jtitle><stitle>Biotechnol Bioproc E</stitle><date>2008-12-01</date><risdate>2008</risdate><volume>13</volume><issue>6</issue><spage>690</spage><epage>696</epage><pages>690-696</pages><issn>1226-8372</issn><eissn>1976-3816</eissn><abstract>The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agitation and aeration during cultivation had a positive effect regarding viability of the bottom-fermenting strains, Rh and Frank. For effective preservation, it was necessary to harvest yeast cells from the stationary phase during cultivation. For lyophilization preservation, the yeast strains tested showed a negative effect on viability, independent of strain or cultivation method. No significant correlation was found between trehalose concentration and yeast viability following either filter paper or lyophilization preservation. However, the filter paper preservation method was suitable for both bottom and top brewing yeast strains with regard to feasibility, viability, and maintenance of the yeast's specific character.</abstract><cop>Heidelberg</cop><pub>The Korean Society for Biotechnology and Bioengineering</pub><doi>10.1007/s12257-008-0066-8</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1226-8372
ispartof Biotechnology and Bioprocess Engineering, 2008, 13(6), , pp.690-696
issn 1226-8372
1976-3816
language eng
recordid cdi_nrf_kci_oai_kci_go_kr_ARTI_177218
source ABI/INFORM Global; Springer Link
subjects Biotechnology
brewing yeast
Chemistry
Chemistry and Materials Science
Chromatography
Cultivation
Fermentation
filter paper preservation
Industrial and Production Engineering
Metabolism
Methods
Nitrogen
Studies
TREHALOSA
TREHALOSE
VIABILIDAD
VIABILITE
VIABILITY
Yeast
Yeasts
생물공학
title Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T21%3A05%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_nrf_k&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Cultivation%20Conditions%20on%20Trehalose%20Content%20and%20Viability%20of%20Brewing%20Yeast%20Following%20Preservation%20via%20Filter%20Paper%20or%20Lyophilization%20Methods&rft.jtitle=Biotechnology%20and%20bioprocess%20engineering&rft.au=Cheong,%20C.%20(Seoul%20University%20of%20Venture%20and%20Information,%20Seoul,%20Republic%20of%20Korea)&rft.date=2008-12-01&rft.volume=13&rft.issue=6&rft.spage=690&rft.epage=696&rft.pages=690-696&rft.issn=1226-8372&rft.eissn=1976-3816&rft_id=info:doi/10.1007/s12257-008-0066-8&rft_dat=%3Cproquest_nrf_k%3E21298616%3C/proquest_nrf_k%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c401t-91b8298dd2c5455383bcdf07b91d7888160c7ea5d308a7ffd16596931f7ecfe63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=229414768&rft_id=info:pmid/&rfr_iscdi=true