Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract

• Published in: Biotechnology and bioprocess engineering 2009, 14(2), , pp.232-237
• Main Authors: Cha, W.S. (Chosun University, Gwangju, Republic of Korea), Ding, Ji-Lu (Chosun University, Gwangju, Republic of Korea), Choi, D.B. (Chodang University, Muan, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; antitumor activities; Antrodia camphorata; Biotechnology; Chemistry; Chemistry and Materials Science; Comparative analysis; Dehydrogenases; Enzymes; Functional foods & nutraceuticals; Industrial and Production Engineering; Liver cancer; Metabolism; Methanol; nitrite scavenging; Potassium; Studies; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-008-0291-1

The effects of decoctions prepared from Antrodia camphorata on antioxidant, nitrite scavenging, and antitumor activities were investigated. Glutathione (GSH) production was 18.2 μM/g of rat liver under the influence of a methanol extract, a result similar to with the effect of silymarin administration. GSH peroxidase activity was about 2.0-fold higher than with silymarin administration. Superoxide dismutase activity was 18.9 U/mg protein, about 2.5-fold higher than the control group. The hot water extract (1000 ㎍/mL) showed the highest nitrite scavenging activity at 98.1%, similar to those observed with BHA and vitamin E. Among a variety of human cancer cell lines, when the concentration of hot water extract was increased from 31 to 500 ㎍/mL, the viability of Hep3B cell was decreased from 100 to 60.7%. On the other hand, in the case of methanol extract, it was decreased from 98.4 to 10.0%. These results supported the conclusion that the antioxidant, nitrite scavenging, and antitumor activities of this A. camphorata methanol extract indicate a potential source for the development of various health supplements and pharmaceutical and nutraceutical applications.