Enhancement of α-ketoglutarate production in Torulopsis glabrata: Redistribution of carbon flux from pyruvate to α-ketoglutarate

• Published in: Biotechnology and bioprocess engineering 2009, 14(2), , pp.134-139
• Main Authors: Zhang, Dandan (Jiangnan University, Wuxi, China), Liang, Nan (Jiangnan University, Wuxi, China), Shi, Zhongping (Jiangnan University, Wuxi, China), Liu, Liming (Jiangnan University, Wuxi, China), E-mail: [e-mail], Chen, Jian (Jiangnan University, Wuxi, China), E-mail: [e-mail], Du, Guocheng (Jiangnan University, Wuxi, China)
• Format: Article
• Language: English
• Subjects: alpha-ketoglutarate; Biotechnology; Chemistry; Chemistry and Materials Science; Industrial and Production Engineering; PIRUVATOS; PYRUVATE; PYRUVATES; TORULOPSIS GLABRATA; 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-008-0169-2

This manuscript aimed at increasing the production of α-ketoglutarate by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019. The carbon flux was redistributed from pyruvate to α-ketoglutarate node by manipulating the specific activity of pyruvate dehydrogenase complex (PDH), pyruvate carboxylase (PC), pyruvate decarboxylase (PDC), and α-ketoglutarate dehydrogenase complex (KGDH). By proper increase of PDH, PC, and PDC activities, α-ketoglutarate in fermentation broth could be accumulated to the levels of 17.1 g/L, 21.6 g/L, and 31.2 g/L, respectively. In addition, decrease in the specific activity of KGDH also resulted in an enhanced α-ketoglutarate synthesis. With a proposed combinational enzymes regulation strategy, the highest α-ketoglutarate concentration of 37.7 g/L was achieved.