Effects of synthetic lysophosphatidylcholines on suspension cultures of the Chinese hamster ovary DG44 cells in protein-free media

This study described the effects of synthetic lysophosphatidylcholines on the growth of recombinant CHO-DG44 cells in suspension. Overall, cell growth characteristics were improved when cultivated in suspension in a protein-free medium supplemented with natural soybean lysophosphatidylcholines. To s...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2008, 13(4), , pp.396-400
Main Authors: Kim, I.K. (Hanyang University, Seoul, Republic of Korea), Lim, D.J. (Hanyang University, Seoul, Republic of Korea), Park, H.W. (Hanyang University, Seoul, Republic of Korea), E-mail: hhwwpp@chol.com
Format: Article
Language:English
Subjects:
Biosynthesis
Biotechnology
Cell growth
Cellular biology
Chemistry
Chemistry and Materials Science
Chinese hamster ovary cells
CULTURE TECHNIQUES
Dihydrofolate reductase
Experiments
Industrial and Production Engineering
Lipids
lysophosphatidylcholines
Ovaries
protein-free medium
Proteins
Soybeans
Studies
TECHNIQUE DE CULTURE
TECNICAS DE CULTIVO
생물공학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study described the effects of synthetic lysophosphatidylcholines on the growth of recombinant CHO-DG44 cells in suspension. Overall, cell growth characteristics were improved when cultivated in suspension in a protein-free medium supplemented with natural soybean lysophosphatidylcholines. To substitute synthetic lysophosphatidylcholines for the naturally occurring lysophosphatidylcholines, we implemented a systematic approach in which twelve synthetic lysophosphatidylcholines were grouped into three lipid mixtures according to the length of their acyl chains. We found that synthetic lysophosphatidylcholines with medium acyl chain lengths (C14-C18), including oleoyl lysophosphatidylcholine (C18:1) could increase cell growth in the protein-free media. The fortified protein-free medium with medium acyl chain length lysophosphatidylcholines (C14-C18) maintained growth of CHO-DG44 cells over five consecutive passages, whereas the cell growth in a CHO protein-free medium was decreased gradually after four passages. We also observed that the restorative effect of oleoyl lysophosphatidylcholine was comparable to that of natural lysophosphatidylcholine in batch and long-term cultivation. These results show that synthetic lysophosphatidylcholines can be used as lipid supplements in either protein-free media or chemically defined media for CHO cell suspension cultures.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-008-0148-7