Enantioselective Hydrolysis of Racemic Styrene Oxide by Epoxide Hydrolase of Rhodosporidium kratochvilovae SYU-08

Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethan...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2003, 8(5), , pp.306-308
Main Authors: Lee, J W, Lee, E J, Yoo, S S, Park, SH, Kim, H S, Lee, E Y
Format: Article
Language:English
Subjects:
Rhodosporidium kratochvilovae
생물공학
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanediol concentrations on hydrolysis rate, and enantioselectivity - were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate, with a yield of 38 % (theoretically 50% maximum yield) from an initial concentration of 80 mM.
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02949223