Optimization of γ-Polyglutamic Acid Production by Bacillus subtilis ZJU-7 Using a Surface-response Methodology

The components of the media used to elicit the biosynthesis of poly-γ-glutamic acid (γ-PGA) by Bacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources. Of the 7 carbon sources investigated, sucrose induced the highest rate of γ-PGA productivity; among the nitrogen sou...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2006, 11(3), , pp.251-257
Main Authors: Zhinan Xu, Feng Shi, Peilin Cen
Format: Article
Language:Korean
Subjects:
생물공학
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Summary:The components of the media used to elicit the biosynthesis of poly-γ-glutamic acid (γ-PGA) by Bacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources. Of the 7 carbon sources investigated, sucrose induced the highest rate of γ-PGA productivity; among the nitrogen sources, tryptone had the best effect for γ-PGA production. A 26-2 fractional factorial design was used to screen factors that influence γ-PGA production significantly, and a central composite design was finally adopted to formulate the optimal medium. γ-PGA productivity improved approximately 2-fold when the optimal medium was used compared with the original nonoptimized medium, and volumetric productivity reached a maximum of 58.2 g/L after a 24-h cultivation period. KCI Citation Count: 36
ISSN:1226-8372
1976-3816