Genes related with apoptosis by inflammation in diabetic keratocytes

• Published in: Genes & genomics 2015, 37(7), , pp.607-614
• Main Authors: Park, Y.M., Pusan National University Yangsan Hospital, Yangsan, Republic of Korea, Lee, J.E., Pusan National University Yangsan Hospital, Yangsan, Republic of Korea, Kim, C.D., Pusan National University College of Medicine, and MRC for Ischemic Tissue Regeneration, Yangsan, Republic of Korea, Lee, J.S., Pusan National University College of Medicine, and MRC for Ischemic Tissue Regeneration, Yangsan, Republic of Korea
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; APOPTOSE; APOPTOSIS; Biomedical and Life Sciences; Human Genetics; IL-1alpha,Keratocyte,Microarray,TNF-alpha; INFLAMACION; INFLAMMATION; Life Sciences; Microbial Genetics and Genomics; Plant Genetics and Genomics; Research Article; 생물학
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-015-0290-5

The goal of this study was to identify the differences in apoptosis-related gene expression between normal and diabetic keratocytes stimulated with IL-1a and TNF-alpha. Primary cultured normal and diabetic keratocytes were treated with IL-1a and TNF-alpha. The cDNA from these cells was hybridized to an oligonucleotide microarray. Microarray analysis was used to identify differentially expressed genes, which were further evaluated by real-time polymerase chain reaction. Diabetic keratocytes overexpressed vital components of cell division, migration, and differentiation, including Ppm1a, Ncam1, and Capn9, and under-expressed components related to prevention of apoptosis, including Clu, Ptn, and Mycn. Genes commonly over-expressed in response to IL-1alpha and TNF-alpha include Siah1a, Gzmb, and Capn9. Genes underexpressed after treatment with both cytokine include Ets1, Igf1r, and Sgk. Eighteen and eight apoptosis-related genes were newly expressed after treatment with IL-1alpha and TNF-alpha, respectively. The genes commonly under-expressed after treatment with both cytokines were Bmp4 and Timp3. Real-time polymerase chain reaction showed results similar to those of the microarray analysis. IL-1alpha and TNF-alpha may modulate the expression of genes related to apoptosis in diabetic keratocytes. It may be helpful to suppress newly overexpressed genes or to enhance newly underexpressed genes by IL-1alpha or TNF-alpha for the prevention of apoptosis in keratocytes when the cornea is damaged.