In vitro CpG methylation and garcinol reduce PERV LTR promoter activity

Porcine endogenous retroviruses (PERVs) in the pig genome represent a potential risk of infection in pig-to-human transplantation. Long terminal repeats (LTRs) are known to be strong promoter elements that could regulate the transcription activity of PERV elements. It is possible that DNA methylatio...

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Published in:Genes & genomics 2012, 34(2), , pp.217-222
Main Authors: Ha, H.S., Pusan National University, Busan, Republic of Korea, Lee, Y.C., Dong-A University, Busan, Republic of Korea, Park, S.J., Pusan National University, Busan, Republic of Korea, Jung, Y.D., Pusan National University, Busan, Republic of Korea, Ahn, K., Pusan National University, Busan, Republic of Korea, Moon, J.W., Pusan National University, Busan, Republic of Korea, Han, K.D., Dankook University, Cheonan, Republic of Korea, Oh, K.B., National Livestock Research Institute, RDA, Suwon, Republic of Korea, Kim, T.H., National Livestock Research Institute, RDA, Suwon, Republic of Korea, Seong, H.H., National Livestock Research Institute, RDA, Suwon, Republic of Korea, Kim, H.S., Pusan National University, Busan, Republic of Korea
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
CpG islands
DNA methylation
Human Genetics
JABALI
Life Sciences
LTR
Microbial Genetics and Genomics
PERV
Plant Genetics and Genomics
Research Article
SANGLIER
WILD BOAR
생물학
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Summary:Porcine endogenous retroviruses (PERVs) in the pig genome represent a potential risk of infection in pig-to-human transplantation. Long terminal repeats (LTRs) are known to be strong promoter elements that could regulate the transcription activity of PERV elements. It is possible that DNA methylation controls promoter activity of PERV family. Here, we analyzed CpG dinucleotides and CpG islands of six transcribed PERV LTRs. Promoter activity of the LTRs from the six clones methylated by CpG methyltransferase (M. SssI), and luciferase assay after garcinol treatment (histone acetyltransferase inhibitor) were examined, indicating that promoter activity of the PERV LTRs was significantly decreased.
ISSN:1976-9571
2092-9293
DOI:10.1007/s13258-011-0161-7