Analysis of gene expression profiles from subcutaneous adipose tissue of two pig breeds

Lipid deposition, especially in subcutaneous (backfat) adipose tissues, is directly related to the meat yield and quality in pork. We hypothesized that differentially expressed genes and their transcription factors play a role in the regulation of adipogenesis in porcine backfat. We identified 136 d...

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Bibliographic Details
Published in:Genes & genomics 2011, 33(6), , pp.693-699
Main Authors: Lim, D.J., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Lee, K.T., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Park, J.E., Seoul National University, Seoul, Republic of Korea, Kim, H.B., Seoul National University, Seoul, Republic of Korea, Kim, T.H., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Choi, B.H., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Kim, M.J., National Institute of Animal Science, RDA, Cheonan, Republic of Korea, Park, H.S., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Jang, G.W., National Institute of Animal Science, RDA, Suwon, Republic of Korea
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
BACKFAT
Biomedical and Life Sciences
cDNA microarray
Differentiallly Expressed genes
Human Genetics
LARD DORSAL
Life Sciences
Microbial Genetics and Genomics
Plant Genetics and Genomics
Porcine
Research Article
TOCINO DORSAL
생물학
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Summary:Lipid deposition, especially in subcutaneous (backfat) adipose tissues, is directly related to the meat yield and quality in pork. We hypothesized that differentially expressed genes and their transcription factors play a role in the regulation of adipogenesis in porcine backfat. We identified 136 differentially expressed genes (DEGs) in subcutaneous adipose tissue between two pig breeds, the Korean native pig (KNP) and Yorkshire, using a cDNA microarray. Gene ontology analysis demonstrated that some DEGs are involved in fat metabolism processes such as adipogenesis, adipocyte differentiation, and lipolysis. In addition, we identified the regulatory relationship between DEGs and their transcription factors using in silico and qRT-PCR analysis. Two transcription factors (TEF-1, IRF-7) have positively regulated DEGs in KNP backfat. Three other transcription factors (STAT3, OCT1 and HNF4) are negatively correlated with the DEGs in KNP backfat. Our findings suggest that DEGs and their transcription factors may have a potential role in adipogenesis and/or lipid deposition in backfat tissues between pig breeds.
ISSN:1976-9571
2092-9293
DOI:10.1007/s13258-011-0083-4