Characterization and expression pattern of IbPRP1 and IbPRP2 stress-related genes from sweetpotato

Two putative stress-related genes were isolated from sweetpotato and were designated as IbPRP1 and IbPRP2 (Ipomoea batatas proline-rich proteins 1 and 2). The deduced amino acid aligment of IbPRP1 and IbPRP2 shows that these two genes belong to the AAI_LTSS superfamily. Proteins in this family are k...

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Published in:Genes & genomics 2010, 32(5), , pp.487-497
Main Authors: Seo, S.G., The University of Seoul, Seoul, Republic of Korea, Jeon, S.B., The University of Seoul, Seoul, Republic of Korea, Kim, J.S., The University of Seoul, Seoul, Republic of Korea, Shin, J.M., The University of Seoul, Seoul, Republic of Korea, Kim, J.B., Konkuk University, Chungju, Republic of Korea, Kang, S.W., Chung-Ang University, Ansung, Republic of Korea, Lee, G.P., Chung-Ang University, Ansung, Republic of Korea, Kim, S.H., The University of Seoul, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
BATATA
Biomedical and Life Sciences
Cell wall protein
Human Genetics
Life Sciences
Microbial Genetics and Genomics
PATATE DOUCE
Plant Genetics and Genomics
Proline-rich Protein (PRP)
Research Article
SWEET POTATOES
생물학
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Summary:Two putative stress-related genes were isolated from sweetpotato and were designated as IbPRP1 and IbPRP2 (Ipomoea batatas proline-rich proteins 1 and 2). The deduced amino acid aligment of IbPRP1 and IbPRP2 shows that these two genes belong to the AAI_LTSS superfamily. Proteins in this family are known to play primary roles including defending plants from pathogens and insects, lipid transport between intracellular membranes, and nutrient storage. The mRNA expression of IbPRP1 and IbPRP2 were investigated and the results demonstrate that IbPRP2 has tissue-specific expression. Moreover, IbPRP1 and IbPRP2 may be involved in response to various stresses including drought, pathogen, and oxidative stress. In addition, when leaf disc test was performed, the IbPRP1 transgenic tobacco plants showed increase in tolerance to salt (100 mM, 200 mM, and 300 mM). Moreover, IbPRP1 and IbPRP2 may have some roles of transmembrane protein in sweetpotato when checked through GFP fusion cell localization and transmembrane analysis. All of these results indicate that IbPRP1 and IbPRP2 might play an important role in plant stress responses.
ISSN:1976-9571
2092-9293
DOI:10.1007/s13258-010-0072-z