Molecular cloning of novel α-gliadin genes from Crithopsis delileana and the evolution analysis with those from Triticeae

• Published in: Genes & genomics 2011, 33(2), , pp.155-161
• Main Authors: Guo, Zhi-Fu, Shenyang Agricultural University, Shenyang, China, Long, Xiang-Yu, Sichuan Agricultural University, Yaan, Sichuan, China, Dong, Pan, Sichuan Agricultural University, Yaan, Sichuan, China, Wei, Yu-Ming, Sichuan Agricultural University, Yaan, Sichuan, China, Bai, Li-Ping, Shenyang Agricultural University, Shenyang, China, Dang, Xiao-Xuan, Shenyang Agricultural University, Shenyang, China, Wan, Hao-Lei, Chinese Academy of Sciences, Beijing, China, Zhang, Li-Jun, Shenyang Agricultural University, Shenyang, China, Zheng, You-Liang, Sichuan Agricultural University, Yaan, Sichuan, China
• Format: Article
• Language: English
• Subjects: alpha-gliadin gene; Animal Genetics and Genomics; Biomedical and Life Sciences; Crithopsis delileana; GENE; GENES; Human Genetics; Life Sciences; Microbial Genetics and Genomics; Phylogenetic analysis; Plant Genetics and Genomics; Research Article; Triticeae; 생물학
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-010-0128-0

The α-gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) were investigated by Acid polyacrylamide gel electrophoresis (A-PAGE) analysis. It was indicated that the electrophoresis mobility of gliadins from C. delileana had obvious difference with those from common wheat in α, γ and ω region. Using primers designed from published sequences of α-gliadin genes, three α-gliadin genes were isolated from C. delileana, which were designated as gli-ka1, gli-ka2 and gli-ka3, respectively. Two in-frame stop codons were found in the coding sequences of gli-ka3, indicating that gli-ka3 could be a pseudogene. The gli-ka2 was a gliadin with an odd number of cysteines, resulting from a non-synonymous mutation. This change might lead to the interactive behavior of gli-ka2. Three α-gliadin genes of C. delileana had the similar but not identical primary structures to the corresponding gene sequences from other wheat related species. By the alignment of α-gliadin genes from Triticeae, phylogenetic analysis indicated that three α-gliadin genes of C. delileana clustered together with all α-gliadin genes from Ee genome of Lophopyrum elongatum by an interior paralleled branch.