Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging

• Published in: Laboratory animal research 2015, 31(3), , pp.148-152
• Main Authors: Choi, E.K., Chungbuk National University, Cheongju, Republic of Korea, Guo, H., Chungbuk National University, Cheongju, Republic of Korea, Choi, J.K., Dr. Chungs Food Co. Ltd., Eumseong, Republic of Korea, Jang, S.K., Gangneung-Wonju National University, Gangneung, Republic of Korea, Shin, K., Chungbuk National University, Cheongju, Republic of Korea, Cha, Y.S., Chungbuk National University, Cheongju, Republic of Korea, Choi, Y., Chungbuk National University, Cheongju, Republic of Korea, Seo, D.W., Chungbuk National University, Cheongju, Republic of Korea, Lee, Y.B., Dr. Chungs Food Co. Ltd., Eumseong, Republic of Korea, Joo, S.S., Gangneung-Wonju National University, Gangneung, Republic of Korea, Kim, Y.B., Chungbuk National University, Cheongju, Republic of Korea
• Format: Article
• Language: English
• Subjects: Letter; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Rosa hybrida,white rose petal extract,matrix metalloproteinase-1,elastase; 수의학
• ISSN: 1738-6055; 2233-7660
• DOI: 10.5625/lar.2015.31.3.148

In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 micro gram/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 microgram/mL. High concentrations (¡Ã250 micro gram/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 micro gram/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 micro mole, reaching 80% inhibition at 125 micro mole. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.