Determination of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector

This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C∧18, 4.6×250 mm, 5 ㎛) with a mobile phase...

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Published in:Laboratory animal research 2011, 27(1), , pp.37-40
Main Authors: Jo, K., Konkuk University, Seoul, Republic of Korea, Cho, H.J., Konkuk University, Seoul, Republic of Korea, Yi, H., Konkuk University, Seoul, Republic of Korea, Cho, S.M., Konkuk University, Seoul, Republic of Korea, Park, J.A., Konkuk University, Seoul, Republic of Korea, Kwon, C.H., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea, Park, H.R., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea, Kwon, K.S., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea, Shin, H.C., Konkuk University, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
ANTHELMINTHIQUE
ANTHELMINTICS
ANTIHELMINTICOS
BEEF
CARNE DE RES
Determination
HPLC
LAIT
LECHE
MILK
Original
VIANDE BOVINE
수의학
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Summary:This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C∧18, 4.6×250 mm, 5 ㎛) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (60:40, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acid in acetonitrile for commercial milk, dehydration by adding sodium sulfate to the liquid analytical sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 ㎛ syringe filter. This method had good selectivity and recovery (70.70±7.90-110.79±14.95%) from the matrices. The LOQs ranged from 9.7 to 9.8 ㎍/kg for beef and commercial milk. The recoveries met the standards set by the CODEX guideline.
ISSN:1738-6055
2233-7660
DOI:10.5625/lar.2011.27.1.37