miRNA-mediated expression switch of cell adhesion genes driven by microcirculation in chip

• Published in: Biochip journal 2017, 11(4), , pp.262-271
• Main Authors: Samatov, Timur R., Galatenko, Vladimir V., Senyavina, Nadezhda V., Galatenko, Alexey V., Shkurnikov, Maxim Yu, Tonevitskaya, Svetlana A., Sakharov, Dmitry A., Marx, Uwe, Ehrlich, Hermann, Schumacher, Udo, Tonevitsky, Alexander G.
• Format: Article
• Language: English
• Subjects: Biomedical Engineering and Bioengineering; Biotechnology; CD44 antigen; Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Cell culture; Chemistry; Chemistry and Materials Science; Colon; Colon cancer; Colorectal cancer; Connexin 43; CYR61 protein; Epithelium; Gap junctions; Gene expression; Genes; Intestine; Microenvironments; Microfluidics; MicroRNAs; miRNA; Original Article; 생물공학
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-017-1305-x

Changes in cell adhesion molecule (CAM) expression and miRNAs regulating them are known to be involved in malignant progression in colon cancer. We investigated expression profiles of CAM genes and non-coding RNAs in CaCo2 colon cancer cells in static culture and under dynamic flow conditions perfused in microfluidic chip emulating physiological microenvironment. We incubated monolayers of CaCo2 cells in Transwell® units either under static conditions or under flow in a microfluidic chip. We identified 7 up-regulated CAM genes ( CD44 , CDH7 , CEACAM5 , CEACAM6 , CYR61 , L1CAM and VCAN ), 7 down-regulated genes ( COL12A1 , FGA , FGB , FGG , GJA1 , ITGA5 and LAMA1 ) and 69 miRNAs targeting them under the influence of microcirculation. The revealed network comprised CAM genes known to interact with each other and 13 miRNAs simultaneously regulating more than one of them. The discovered regulatory network comprising CAM genes and miRNAs is likely involved in normal functioning of intestine epithelium as well as in cancer progression.