Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy

Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high through...

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Bibliographic Details
Published in:The plant pathology journal 2013, 29(1), , pp.105-109
Main Authors: Kim, Saetbyeol, Lee, Sanguk, Chi, Hee-Youn, Kim, Mi-Kyeong, Kim, Jeong-Soo, Lee, Su-Heon, Chung, Hoeil
Format: Article
Language:English
Subjects:
discrimination
Raman spectroscopy
surface enhanced Raman scattering (SERS)
Turnip yellow mosaic virus (TYMV)
virus detection
농학
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Summary:Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique.
ISSN:1598-2254
2093-9280
DOI:10.5423/PPJ.NT.09.2012.0147