Overexpression of stearoyl-ACP desaturase enhances accumulations of oleic acid in the green alga Chlamydomonas reinhardtii

FAB2, which encodes stearoyl-acyl carrier protein desaturase, catalyzes the conversion of stearic acid (18:0) to oleic acid (18:1) in fatty acid biosynthesis. In this study, we isolated FAB2 from Chlamydomonas reinhardtii, named CrFAB2, and generated CrFAB2-overexpressing transgenic lines to identif...

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Published in:Plant biotechnology reports 2014, 8(2), , pp.135-142
Main Authors: Hwangbo, Kwon, Ahn, Joon-Woo, Lim, Jong-Min, Park, Youn-Il, Liu, Jang Ryol, Jeong, Won-Joong
Format: Article
Language:English
Subjects:
Agriculture
Algae
Analysis
Aquatic plants
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cell Biology
Chlamydomonas reinhardtii
Chlorophyll
Enzymes
fatty acid composition
Fatty acids
Gene expression
gene overexpression
Genomes
Life Sciences
linoleic acid
oleic acid
Original Article
Plant Biochemistry
Plant Sciences
Plastids
Proteins
stearic acid
Studies
농학
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Summary:FAB2, which encodes stearoyl-acyl carrier protein desaturase, catalyzes the conversion of stearic acid (18:0) to oleic acid (18:1) in fatty acid biosynthesis. In this study, we isolated FAB2 from Chlamydomonas reinhardtii, named CrFAB2, and generated CrFAB2-overexpressing transgenic lines to identify a major role of CrFAB2 in fatty acid biosynthesis of C. reinhardtii. In CrFAB2-overexpressing lines, oleic acid (18:1) content was increased by approximately 2.4-fold compared to the wild-type control plants. Interestingly, CrFAB2 overexpression resulted in the induction of CrFAD2 expression. Consistent with this result, the induction of linoleic acid (18:2) was also detected in CrFAB2-overexpressing lines, and total fatty acid content in these lines was induced by approximately 28 % by CrFAB2 overexpression compared to the wild-type control. Our results indicate that CrFAB2 overexpression enhances the synthesis of oleic acid (18:1) and that CrFAB2 may also play a key role in regulating total fatty acid content in the green alga C. reinhardtii.
ISSN:1863-5466
1863-5474
DOI:10.1007/s11816-013-0302-3