C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes

WRKY proteins are known to play major roles in defense signaling. We identified a pathogen-inducible and SA-inducible OsWRKY30. Our cDNA clone encodes the C-terminal region (CTR) of OsWRKY30. CTR-OsWRKY30 includes the C-terminal WRKY domain and nuclear localization sequence. CTR-OsWRKY30 was suffici...

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Bibliographic Details
Published in:Plant biotechnology reports 2013, 7(3), , pp.221-230
Main Authors: Lee, Heyoung, Ko, Yu Jin, Cha, Joo-Young, Park, Sang Ryeol, Ahn, Ilpyung, Hwang, Duk-Ju
Format: Article
Language:English
Subjects:
Agriculture
Arabidopsis
Binding sites
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cloning
complementary DNA
Flowers & plants
Gene banks
gene expression
genes
Leaves
Life Sciences
nuclear localization signals
Original Article
Overexpression
Pathogens
Plant Biochemistry
Plant biology
Plant Sciences
rice
Studies
Transcription factors
Transgenic plants
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Summary:WRKY proteins are known to play major roles in defense signaling. We identified a pathogen-inducible and SA-inducible OsWRKY30. Our cDNA clone encodes the C-terminal region (CTR) of OsWRKY30. CTR-OsWRKY30 includes the C-terminal WRKY domain and nuclear localization sequence. CTR-OsWRKY30 was sufficient to bind W-box sequences (TTGACC/T). Over-expression of the CTR-OsWRKY30 resulted in enhanced resistance to pathogens in Arabidopsis and rice. Defense-related genes were constitutively expressed in transgenic Arabidopsis and rice over-expressing CTR-OsWRKY30. Based on promoter transient assays, CTR-OsWRKY30 is sufficient to activate OsPR10a promoter as much as full length OsWRKY30. Taken together, CTR-OsWRKY30 positively regulates defense signaling, thereby resulting in enhanced resistance to pathogens.
ISSN:1863-5466
1863-5474
DOI:10.1007/s11816-012-0252-1