Transgenic overexpression of UIP1, an interactor of the 30 untranslated region of the Rubisco small subunit mRNA, increases rice tolerance to drought

Gene regulation at the post-transcriptional level is a well-organized process to adjust plants in response to environmental changes. Here, we identified a novel RNAbinding protein (RBP) possessing a CBS (cystathionine-bsynthase) domain through yeast three-hybrid screening. This RBP, 30-UTR-interacti...

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Published in:Plant biotechnology reports 2013, 7(1), , pp.83-90
Main Authors: 박수현, Jin Seo Jeong, Mark C. F. R. Redillas, Harin Jung, Seung Woon Bang, Youn Shic Kim, 김주곤
Format: Article
Language:English
Subjects:
농학
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Summary:Gene regulation at the post-transcriptional level is a well-organized process to adjust plants in response to environmental changes. Here, we identified a novel RNAbinding protein (RBP) possessing a CBS (cystathionine-bsynthase) domain through yeast three-hybrid screening. This RBP, 30-UTR-interacting protein 1 (UIP1), interacts with 30 untranslated region of the Rubisco small subunit mRNA (30 RbcS)—the major mRNA element that mediates the stress-induced mRNA decay (SMD) under drought and salt stress conditions. Six deletion constructs were made to delineate the binding domain of the UIP1 protein. Cotransformation of yeast with these constructs together withthree different hybrid RNAs in various combinations showed that deletion of 51 N-terminal amino acids resulted in a loss of sequence-specific binding affinity. Further deletion at the region between 52 and 212 amino acids revealed that the CBS domain of UIP1 is necessary for binding to 30 RbcS. Transgenic overexpression of UIP1 in rice resulted in an increase in tolerance to drought stress at the vegetative stage of growth. Under drought, high salt and low temperature conditions, the maximum photochemical efficiency of photosystem II (Fv/Fm) of UIP1 plants was higher than those of the nontransgenic plants. Interestingly, the effect of UIP1 overexpression on tolerance to stress was much more pronounced under drought than under high salt and low temperature conditions. Taken together, our results demonstrate that UIP1 interacts with 30 untranslated region of RbcS1 mRNA and increases tolerance of transgenic overexpressors to drought stress. KCI Citation Count: 7
ISSN:1863-5466
1863-5474