A novel WD40 protein, BnSWD1, is involved in salt stress in Brassica napus

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant ( Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1–5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nuc...

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Published in:Plant biotechnology reports 2010, 4(2), , pp.165-172
Main Authors: Lee, Sanghun, Lee, Junhee, Paek, Kyung-Hee, Kwon, Suk-Yoon, Cho, Hye Sun, Kim, Shin Je, Park, Jeong Mee
Format: Article
Language:English
Subjects:
Abiotic stress
Abscisic acid
Agriculture
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cloning
Enzymes
Gene expression
Genes
Kinases
Life Sciences
Plant Biochemistry
Plant Sciences
Proteins
Rape plants
Salinity
Salt
Salts
Seeds
Short Communication
Signal transduction
Sodium chloride
Stress response
Studies
Tomatoes
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Summary:Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant ( Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1–5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of β-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 ( Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.
ISSN:1863-5466
1863-5474
DOI:10.1007/s11816-010-0131-6