HPLC–UV analysis of sample preparation influence on flavonoid yield from Cirsium japonicum var. maackii

This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed....

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Bibliographic Details
Published in:Applied biological chemistry 2017, 60(5), , pp.519-525
Main Authors: Rodriguez, Joyce P., Lee, Jaemin, Park, Jun Yeon, Kang, Ki Sung, Hahm, Dae-Hyun, Lee, Sang Cheon, Lee, Sanghyun
Format: Article
Language:English
Subjects:
Applied Microbiology
Biological Techniques
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Cirsium
Ethanol
Flavonoids
High-performance liquid chromatography
Liquid chromatography
Optimization
Sample preparation
Solvents
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Summary:This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed. Three flavonoids were specifically investigated: cirsimarin ( 1 ), hispidulin ( 2 ), and cirsimaritin ( 3 ). In single-factor experiments, each variable had a significant effect on the determination of content of compounds 1 – 3 . The optimal conditions for extraction were found to be: mass, 15 g; ratio of spring and fall leaves, 4:1; extraction solvent, 70% ethanol; extraction time, 4 h; solid-to-solvent ratio, 1:20; and number of extractions, 1. The results of the study were used to maximize the potential of ICF-1 samples and optimize the efficiency of the extraction process.
ISSN:2468-0834
2468-0842
DOI:10.1007/s13765-017-0306-8