Focusing and sorting of multiple-sized beads and cells using low-aspect-ratio spiral microchannels

A separation of Polystyrene micro-beads (PMBs) and natural Mast-cell tumor cells (MCTCs) of about 10–25 μm diameter was performed using a microfluidic spiral microchannel. In this study, five-turned spiral microchannels were 500 μm high and 130 μm wide with an initial radius of curvature of 5.5 mm....

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Bibliographic Details
Published in:Journal of mechanical science and technology 2017, 31(11), , pp.5397-5405
Main Authors: Thanormsridetchai, Attawut, Ketpun, Dettachai, Srituravanich, Werayut, Piyaviriyakul, Prapruddee, Sailasuta, Achariya, Jeamsaksiri, Wutthinan, Sripumkhai, Witsaroot, Pimpin, Alongkorn
Format: Article
Language:English
Subjects:
Beads
Control
Crack propagation
Curvature
Dynamical Systems
Engineering
Flow velocity
Grain boundaries
Industrial and Production Engineering
Low aspect ratio
Mechanical Engineering
Microchannels
Polystyrene resins
Propagation
Radius of curvature
Vibration
기계공학
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Summary:A separation of Polystyrene micro-beads (PMBs) and natural Mast-cell tumor cells (MCTCs) of about 10–25 μm diameter was performed using a microfluidic spiral microchannel. In this study, five-turned spiral microchannels were 500 μm high and 130 μm wide with an initial radius of curvature of 5.5 mm. Sorting efficacy of targeted PMB or MCTC samples of each size by focusing them into a single stream depended on the size of the samples and the flow rate. Flow cytometric analysis revealed that almost 90 % of PMBs and MCTCs of 15–25 μm diameter could be successfully sorted from a mixture of smaller beads or cells at a flow rate of 1.0 mL/min.
ISSN:1738-494X
1976-3824
DOI:10.1007/s12206-017-1034-z