Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum

A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, huma...

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Bibliographic Details
Published in:Biochip journal 2018, 12(1), , pp.69-74
Main Authors: Lee, SangWook, Laurell, Thomas, Jeong, Ok Chan, Kim, Soyoun
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Biomarkers
Biomedical engineering
Biomedical Engineering and Bioengineering
Biomedical Laboratory Science/Technology
Biomedicinsk laboratorievetenskap/teknologi
Biotechnology
Chemistry
Chemistry and Materials Science
Immunoassay
Macro/nano-structure silicon
Medical and Health Sciences
Medical Biotechnology
Medicin och hälsovetenskap
Medicinsk bioteknologi
Nanocomposites
Optical activity
Original Article
Prostate cancer
Prostate-specific antigen
Protein arrays
Proteins
Reverse-phase assay
Silicon
Sol-gel microarray
Sol-gel processes
생물공학
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Summary:A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 10 3 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-017-2109-8