In vitro development of canine somatic cell nuclear transfer embryos in different culture media

The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 se...

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Bibliographic Details
Published in:Journal of veterinary science (Suwŏn-si, Korea) 2015, 16(2), , pp.233-235
Main Authors: Kim, Dong-Hoon, No, Jin-Gu, Choi, Mi-Kyung, Yeom, Dong-Hyeon, Kim, Dong-Kyo, Yang, Byoung-Chul, Yoo, Jae Gyu, Kim, Min Kyu, Kim, Hong-Tea
Format: Article
Language:English
Subjects:
Animals
Blastocyst - cytology
Cloning, Organism - veterinary
Culture Media - metabolism
Dogs - embryology
Embryo Culture Techniques - veterinary
Embryonic Development
Nuclear Transfer Techniques - veterinary
Short Communication
수의학
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Summary:The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
ISSN:1229-845X
1976-555X
DOI:10.4142/jvs.2015.16.2.233